Amino acid

PepForm Leucine peptides

Also known as: leucine peptide, PepForm Leucine, Leu-Ile-Lys, branched-chain amino acid peptide, BCAA peptide

Overview

PepForm Leucine peptides are bioactive peptide forms of the essential branched-chain amino acid (BCAA) leucine, potentially offering enhanced bioavailability and targeted physiological effects compared to free leucine. Research suggests that specific leucine-containing peptides, such as the tripeptide Leu-Ile-Lys, may exert protective effects in experimental nephrolithiasis by reducing oxidative stress and preserving renal tubular function, as demonstrated in a rat model of oxalate kidney stones [1]. While free leucine supplementation has been studied for roles in muscle protein synthesis, metabolic regulation, and liver health, results are mixed. Leucine supplementation did not prevent weight gain or improve glycemic control in high-fat-fed rats [3], though systematic review data indicate potential benefits on glucose homeostasis in rodent models of glucose intolerance when administered at 90–140 mg/day during metabolic disease development [7]. Leucine also supports protein metabolism and may benefit liver disease patients with BCAA deficiencies, commonly seen in advanced liver disease [8]. However, evidence for PepForm specifically remains limited to preclinical models, and human data are lacking.

Dosage Guide

Therapeutic Doses

For treatment of specific conditions

Exercise recovery250 mg/kg body weightDivided pre-, during, and post-exercise; based on human resistance exercise study
Metabolic support in glucose intolerance (preclinical)90-140 mg/dayIn rodent models; human equivalent dose not established
Renal protection (experimental)11.5 mg/kgLeu-Ile-Lys tripeptide in rat nephrolithiasis model; human relevance unknown

Special Forms

Alternative forms for specific needs

Leu-Ile-Lys tripeptide

Investigational form for reducing oxidative stress in kidney stone models

Free L-leucine

Common supplemental form for muscle and metabolic support

Clinical Notes

  • Human efficacy data for PepForm Leucine peptides are lacking; current evidence is primarily preclinical.
  • High-dose leucine may disrupt balance of other BCAAs and aromatic amino acids, particularly in liver disease.
  • Leucine supplements should be used cautiously in individuals with maple syrup urine disease (MSUD).
  • Effects on glucose metabolism are inconsistent; leucine may not benefit all metabolic conditions.

Research

Key FindingsPubMed
1

Leu-Ile-Lys tripeptide reduced oxidative stress and γ-glutamyltransferase activity in a rat model of oxalate nephrolithiasis.

Effect of Tripeptide Leu-Ile-Lys on the Course of Experimental Nephrolithiasis.
Bulletin of experimental biology and medicine2019
2

Acidic peptides with specific motifs modify calcite crystal growth in vitro, suggesting potential for influencing mineralization processes.

Acidic peptides acting as growth modifiers of calcite crystals.
Chemical communications (Cambridge, England)2004
3

Leucine supplementation did not prevent weight gain or improve glycemic regulation in male rats on a high-fat diet.

Leucine supplementation at the onset of high-fat feeding does not prevent weight gain or improve glycemic regulation in male Sprague-Dawley rats.
Journal of physiology and biochemistry2016
4

Protein substitutes in PKU require adequate dosing and timing to maintain blood phenylalanine control, relevant for amino acid formulation strategies.

Protein substitutes for PKU: what's new?
Journal of inherited metabolic disease2004
5

Supplementing leucine in low-protein diets improved growth performance in piglets, indicating its role as a limiting amino acid in certain diets.

Response of piglets to the standardized ileal digestible isoleucine, histidine and leucine supply in cereal-soybean meal-based diets.
Animal : an international journal of animal bioscience2013
6

Leucine supplementation (250 mg/kg) attenuated muscle damage markers after eccentric exercise in untrained men.

Effect of leucine supplementation on indices of muscle damage following drop jumps and resistance exercise.
Amino acids2012
7

Systematic review found 90–140 mg/day leucine improved metabolic markers in diet-induced obese rodent models when given early in disease progression.

Does L-leucine supplementation cause any effect on glucose homeostasis in rodent models of glucose intolerance? A systematic review.
Amino acids2018
8

BCAAs, including leucine, support hepatocyte function and are used therapeutically in chronic liver disease to correct amino acid imbalances.

Branched-chain amino acids in liver diseases.
World journal of gastroenterology2013

Products Containing PepForm Leucine peptides(1 report)