Amino acid

Carnosine

Also known as: β-alanyl-L-histidine, carnosine dipeptide, L-carnosine

Overview

Carnosine (β-alanyl-L-histidine) is an endogenous dipeptide primarily found in skeletal muscle and brain tissue, with multifunctional roles including antioxidant activity, anti-inflammatory effects, pH buffering, and protection against glycation and oxidative damage. Supplementation with carnosine or its precursor β-alanine has been shown to increase muscle carnosine concentrations, which may improve high-intensity exercise performance by buffering intracellular acidosis [10][1]. Emerging evidence also supports broader health benefits: carnosine supplementation (2g/day) improved cognitive performance in younger adults in a placebo-controlled trial [9], reduced markers of inflammation and oxidative stress such as CRP and malondialdehyde [1], improved lipid profiles in individuals with dyslipidemia [7], and showed potential in improving glycemic control in prediabetes and type 2 diabetes [5]. Its mechanisms include scavenging reactive oxygen species, modulating immune cell function, and inhibiting advanced glycation end-products [6].

Dosage Guide

Therapeutic Doses

For treatment of specific conditions

Cognitive support2 g/dayFor up to 12 weeks; based on NEAT trial
Exercise performance2-3.2 g/day β-alanineTypically divided into 4 doses to minimize paresthesia
Metabolic health (prediabetes/T2DM)1.6-2 g/day carnosineShown to improve HbA1c and fasting glucose
Dyslipidemia1.5-2 g/day carnosineImproves total cholesterol, LDL, HDL, and triglycerides
Maintenance after loading1.2 g/day β-alanineTo sustain elevated muscle carnosine stores post-loading phase

Special Forms

Alternative forms for specific needs

β-alanine

Precursor to carnosine; more commonly used for muscle carnosine elevation due to better bioavailability

Carnosine peptides (e.g., carnosine, anserine)

Direct supplementation; may offer systemic antioxidant and metabolic benefits

Clinical Notes

  • β-alanine commonly causes paresthesia (tingling) at doses >800 mg per dose; recommend divided dosing or sustained-release formulations
  • Long-term safety of high-dose carnosine supplementation (>2g/day) beyond 12 weeks not fully established
  • Carnosine may interact with carnosinase activity; individuals with low serum carnosinase may have prolonged effects
  • Monitor for gastrointestinal discomfort with high-dose carnosine
  • Most evidence for cognitive and metabolic benefits comes from short-term trials; long-term outcomes require further study

Research

Key FindingsPubMed
1

Carnosine and histidine-containing dipeptides significantly reduce circulating CRP and malondialdehyde, indicating anti-inflammatory and antioxidant effects.

Effects of carnosine and histidine-containing dipeptides on biomarkers of inflammation and oxidative stress: a systematic review and meta-analysis.
Nutrition reviews2024
2

2g/day carnosine for up to 12 weeks improved cognitive performance in younger adults in a randomized controlled trial.

Carnosine supplementation improves cognitive outcomes in younger participants of the NEAT trial.
Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics2025
3

β-alanine supplementation (3.2g/day for 46 days) effectively increases muscle carnosine; maintenance dose of ~1.2g/day sustains elevated levels.

β-Alanine dose for maintaining moderately elevated muscle carnosine levels.
Medicine and science in sports and exercise2014
4

Carnosine acts as an intracellular pH buffer and ergogenic aid, enhancing high-intensity exercise capacity.

Carnosine: from exercise performance to health.
Amino acids2013
5

Carnosine and β-alanine supplementation improved HbA1c and fasting glucose in individuals with prediabetes or type 2 diabetes.

Effect of carnosine or beta-alanine supplementation therapy for prediabetes or type 2 diabetes mellitus: a systematic review and meta-analysis of randomized controlled trials.
BMC endocrine disorders2025
6

Carnosine has multimodal actions including antioxidant, anti-inflammatory, anti-glycation, and immunomodulatory effects.

Unveiling the Hidden Therapeutic Potential of Carnosine, a Molecule with a Multimodal Mechanism of Action: A Position Paper.
Molecules (Basel, Switzerland)2022
7

Carnosine and HCDs significantly reduce total cholesterol, LDL-C, and triglycerides while increasing HDL-C.

β-Alanine Supplementation in Combat Sports: Evaluation of Sports Performance, Perception, and Anthropometric Parameters and Biochemical Markers-A Systematic Review of Clinical Trials.
Nutrients2023
8

Meta-analysis confirms beneficial effects of HCDs on cardiovascular risk factors, particularly lipid profiles.

Carnosine and histidine-containing dipeptides improve dyslipidemia: a systematic review and meta-analysis of randomized controlled trials.
Nutrition reviews2020
9

Cognitive benefits observed in healthy younger adults suggest neuroprotective potential beyond aging populations.

Carnosine supplementation improves cognitive outcomes in younger participants of the NEAT trial.
Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics2025
10

Body mass and sex influence carnosine loading, with lower doses required for maintenance in women and smaller individuals.

β-Alanine dose for maintaining moderately elevated muscle carnosine levels.
Medicine and science in sports and exercise2014

Products Containing Carnosine(1 report)