Amino acid

L-methionine

Also known as: L-methionine, methionine, Met, S-methyl-L-homocysteine

Overview

L-methionine is an essential sulfur-containing amino acid that plays a central role in protein synthesis, methylation reactions, and antioxidant defense. It serves as the precursor to S-adenosylmethionine (SAMe), the primary methyl donor in numerous biochemical pathways, including DNA and histone methylation, which are critical for epigenetic regulation and neuronal development [10][1]. Methionine residues in proteins also act as endogenous antioxidants by scavenging reactive oxygen species through reversible oxidation to methionine sulfoxide, a process regulated by methionine sulfoxide reductases [9]. Adequate methionine intake is vital for liver health, lipid metabolism, and homocysteine regulation, although excessive levels may contribute to hyperhomocysteinemia if cofactors such as vitamins B6, B12, and folic acid are insufficient [10][4]. While direct supplementation of L-methionine is not commonly used in clinical practice, its metabolic derivatives like SAMe are studied for liver support and neurological function.

Dosage Guide

Recommended Daily Allowance

For generally healthy individuals

Adults (≥19 years)1300 mg/day(Estimated average requirement based on body weight (15 mg/kg/day for 70-80 kg adult))
Children (1-13 years)20-30 mg/kg/day(Weight-based estimate from dietary protein requirements)
Pregnant women1500 mg/day(Slight increase due to increased protein and methylation demands)
Breastfeeding women1400 mg/day(Increased metabolic and protein synthesis needs)

Therapeutic Doses

For treatment of specific conditions

Acetaminophen overdose (adjunct)140 mg/kg loading, then 70 mg/kg every 4 hours for 17 dosesN-acetylcysteine is standard; methionine is alternative oral antidote
Liver support (investigational)500-1000 mg/dayLimited evidence; SAMe is preferred form for liver health
Homocystinuria (certain types)500-1000 mg/dayOnly in pyridoxine-responsive cases under medical supervision

Upper Intake Limit

Maximum safe daily intake

3000 mgTolerable upper intake level not firmly established; doses above 2–3 g/day may increase homocysteine without adequate B-vitamins

Special Forms

Alternative forms for specific needs

S-adenosylmethionine (SAMe)

Liver disease, depression, osteoarthritis – active metabolite of methionine

N-acetylmethionine

Potential antioxidant and hepatoprotective agent, less studied

Clinical Notes

  • High-dose methionine may elevate homocysteine, increasing cardiovascular risk, especially in individuals with B-vitamin deficiencies
  • Avoid in patients with homocystinuria or severe liver disease unless under medical supervision
  • Methionine supplementation is contraindicated in individuals with methylenetetrahydrofolate reductase (MTHFR) mutations without concurrent B-vitamin support
  • Oral methionine can be used as an alternative to N-acetylcysteine in early acetaminophen overdose in specific settings
  • Monitor homocysteine levels when using long-term, high-dose methionine

Research

Key FindingsPubMed
1

S-adenosylmethionine (SAMe) shows efficacy and safety in liver diseases, with high-quality evidence supporting its use.

S-Adenosylmethionine (SAMe) for Liver Health: A Systematic Review.
Nutrients2024
2

Methionine acts as an antioxidant via reversible oxidation to methionine sulfoxide, protecting proteins from oxidative damage.

Oxidation of methionine in proteins: roles in antioxidant defense and cellular regulation.
IUBMB life2000
3

Methionine metabolism influences lipid metabolism and produces bioactive compounds like homocysteine, glutathione, and H2S.

Lifetime requirement of the methionine cycle for neuronal development and maintenance.
Current opinion in psychiatry2014
4

Folic acid is a key regulator of homocysteine, which is derived from methionine metabolism and linked to cardiovascular risk.

Folic Acid Supplementation in Patients with Elevated Homocysteine Levels.
Advances in therapy2020
5

Homocysteine-lowering with B vitamins does not consistently reduce cardiovascular events despite lowering homocysteine levels.

Homocysteine-lowering interventions for preventing cardiovascular events.
The Cochrane database of systematic reviews2015
6

DL-methionine and its hydroxy analog are effective dietary sources of methionine in pigs, supporting its bioavailability.

Efficacy of DL-methionine hydroxy analog free acid and DL-methionine as methionine sources for pigs.
Journal of animal science2006
7

Lowering homocysteine with B vitamins did not significantly reduce cardiovascular events in large trials.

Homocysteine-lowering interventions for preventing cardiovascular events.
The Cochrane database of systematic reviews2013
8

Hyperhomocysteinemia is associated with lower HDL and increased cardiovascular risk, modulated by vitamin status.

Sulfur-Containing Amino Acids and Lipid Metabolism.
The Journal of nutrition2020
9

Methionine oxidation plays a regulatory role in cellular redox signaling and protection.

Oxidation of methionine in proteins: roles in antioxidant defense and cellular regulation.
IUBMB life2000
10

Methionine cycle integrity is crucial for nervous system development and maintenance, dependent on folate and B12.

Lifetime requirement of the methionine cycle for neuronal development and maintenance.
Current opinion in psychiatry2014

Products Containing L-methionine(1 report)